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Sunday, October 11, 2020 | History

2 edition of Regulation of epithelial polarisation by [beta]1 integrin-mediated interactions. found in the catalog.

Regulation of epithelial polarisation by [beta]1 integrin-mediated interactions.

Suzanne Marie Spong

Regulation of epithelial polarisation by [beta]1 integrin-mediated interactions.

by Suzanne Marie Spong

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Published by University of Manchester in Manchester .
Written in English


Edition Notes

Thesis (Ph.D.), - University of Manchester, School of Biological Sciences.

ContributionsUniversity of Manchester. School of Biological Sciences.
The Physical Object
Pagination268p.
Number of Pages268
ID Numbers
Open LibraryOL16576147M

Integrins play an important role in cellular matrix interactions requisite for cancer cell adhesion, growth, migration and invasion. In this study, we have investigated the expression of integrin subunits α3, α6, αv and β1 in normal ovaries, benign ovarian tumors and ovarian carcinomas of different pathological grades. The expression of these integrins in ovarian cancer cell lines was also Cited by:   During cutaneous wound healing, keratinocyte proliferation and migration are critical for re-epithelialization. In addition the epidermis secretes growth factors, cytokines, proteases, and matricellular proteins into the wound microenvironment that modify the extracellular matrix and stimulate other wound cells that control the inflammatory response, promote angiogenesis and facilitate tissue Cited by:

For polarization assay in non-traumatic cell migration conditions, transfected cells (1 x 10 5) were seeded into culture insert which was firmed stuck on a 22 × 22 mm glass coverslips, the coverslip was in a well of 6-well plate and incubated overnight. In both conditions, cells were then starved in DMEM/F12 with 1% FBS for 24 by: Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  Talin-1 and kindlin-3 regulate alpha4beta1 integrin-mediated adhesion stabilization, but not G protein-coupled receptor-induced affinity upregulation. J Cited by: If the address matches an existing account you will receive an email with instructions to retrieve your username.


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Regulation of epithelial polarisation by [beta]1 integrin-mediated interactions by Suzanne Marie Spong Download PDF EPUB FB2

The lung develops from tightly orchestrated interactions between opposing layers of epithelial and mesenchymal cells separated by a basement membrane, the components of the epithelial-mesenchymal trophic unit (EMTU). 1 During development these reciprocal interactions are driven by paracrine factors that determine the differentiation state of airway epithelial cells and fibroblasts.

2,3 In the Cited by: INTRODUCTION. Cell migration plays a central role in both normal and pathological processes, including embryonic development, wound healing, inflammation, and tumor metastasis (Trinkaus, ).Integrin-mediated adhesion to the extracellular matrix is a critical regulator of cell migration speed for many cell types, including fibroblasts and by: These findings suggest that epithelial β 1 integrin dysfunction has deleterious consequences in lung epithelium through regulation of innate immunity.

The mechanisms whereby these epithelial-macrophage interactions occur are uncertain, and, perhaps more importantly, the function of β 1 integrin in the adult lung is not by: Regulation of epithelial polarisation by [beta]1 integrin-mediated interactions.

book. The regulation of this pathway appears to be impaired or lost in the tumor cells, suggesting that tumor colony formation occurs by independent mechanisms and that loss of proper integrin-mediated cell-ECM interaction may be critical to breast tumor by: DA Fishman, A Kearns, K ChilukuriMetastatic dissemination of human ovarian epithelial carcinoma is promoted by α2β1-integrin-mediated interaction with type 1 collagen Invas Metast, 18 (), pp.

Cited by: 1. Introduction. Epithelial cells normally form a uniform layer maintained through the regulation of cell-cell interactions and apical-basal polarity by several complexes including cell junctions and polarity proteins such as Par6, atypical Protein Kinase C (aPKC), Scribble (Scrib), and Discs large (Dlg).The loss of these complexes, and subsequently apical-basal polarity, disrupts normal Cited by: 2.

Epithelial polarity – Generating and integrating signals from the ECM with integrins. Cell–matrix interactions and the regulation of epithelial cell polarization. the molecular mechanisms of the αV-integrin-mediated regulation of α2β1-integrins in epithelial cells remain to be by: Integrin a 3 b 1 binds to various ligands such as fibronectin, type IV collagen and laminins (Belkin & Stepp ) and is recognized as a factor that plays a role in the migration of epithelial.

Primary canine and rat oral mucosal epithelial cells were cultured with KCM under a 3T3 feeder layer-free condition. Primary canine oral mucosal epithelial cells exhibited a small, cobblestone-like cell shape, and a higher proliferation than primary rat oral mucosal epithelial cells (n = 3) ().At 11 days of culture, canine cells reached confluency, and kept their confluence more than 13 days Cited by: 2.

Lung fibrosis αv integrin-mediated activation of latent TGFβ. Secreted transforming growth factor beta 1 (TGFβ1) is a major pro-fibrogenic cytokine and a key regulator of fibrosis in multiple organs. Therefore, the molecular pathways that regulate TGFβ1 activity and signaling are attractive targets for novel anti-fibrotic Cited by: The regulation of stem cell behavior and maintenance typically involves the integration of both intrinsic and extrinsic cues.

One such external cue, integrin-mediated cell adhesion to the extracellular matrix, plays an important part in regulating stem cell function and maintenance.

In particular, integrins help define and shape the microenvironment in which stem cells are found: the stem cell Cited by:   In healthy adult epithelial tissues, polarized epithelial cells bonded to the basement membrane are held together through adherens junction complexes, consisting of F-actin, catenins and E-cadherin.

1–3 Under pathological conditions, in response to EMT-inducing signals, the epithelial cells weaken their cell-cell adhesions and lose the apico Cited by: 5. Therefore, integrin-mediated adhesion modulates the decision of life or death.

A role for tumor-matrix interactions in the acquisition of drug resistance has been reported for many cancers including breast cancer. Recent evidence suggests that integrin-mediated adhesion to the ECM may undermine the response of tumors to chemotherapeutic by: The alpha 1-subunit (alpha 1 beta 1 defines a collagen receptor) was present in mesangium and base of all tubule epithelial cells; alpha 2 (collagen) was present in mesangium and in distal but not.

Indeed, mechanical regulation of early development also seems to involve integrin-mediated cell-ECM interactions (Basilicata et alMui et al ). Pluripotency in general appears to be associated with lower levels of cell-ECM interactions compared to differentiated cells, and soft substrates have been shown to favor pluripotency by: 4.

Integrin regulation of cell-cell adhesion during epithelial tubule formation Article in Journal of Cell Science (Pt 5) April with 6 Reads How we measure 'reads'.

Regulation of epithelial wound closure and intercellular adhesion by interaction of AF6 with actin cytoskeleton Article in Journal of Cell Science (Pt 16) September with 10 Reads.

On one hand, studies using either human or murine keratinocytes support a role for α 3 β 1 in regulating front–back polarization and promoting processive migration on laminin, 34,53 which occurs in part through regulation of a FAK/SRC–RAC1 signaling axis. 26,34 In contrast, other studies report that α 3 β 1-deficient keratinocytes Cited by:   Like ZOdeficiency, occludin deletion led to mitotic spindle orientation defects.

Single lumen formation required the occludin OCEL domain, which binds to ZO We conclude that ZO-1–occludin interactions regulate multiple phases of epithelial polarization by providing cell-intrinsic signals that are required for single lumen by: Regulation of epithelial cell growth by ZBP growth arrest and apoptosis through its ability to bind the p21 WAF1 promoter or its ability to form protein-protein interactions with p ZBP protein is elevated in a variety of gastrointestinal cancers as well as the pancreas.

Geng M, Adler G, Menke A. The influence of transforming Cited by:. The alpha 6 beta 4 integrin is a receptor involved in the interaction of epithelial cells with basement membranes. This integrin is unique among the known integrins in that its beta 4 subunit has.Rab25 is up-regulated during polarization in Caco2-BBE cells.

Although recent studies suggested that Rab25 is a tumor suppressor in mice and humans for colon cancer (Nam et al., ), no previous investigations examined the association of Rab25 with polarity and vesicle trafficking in colonic cell investigated the expression of Rab25 in eight different colorectal cancer cell lines.THE ROLE OF INTERACTIONS BETWEEN 4N1K AND CD47 ON INTEGRIN ACTIVATION AND INTEGRIN-MEDIATED CELL ADHESION by Pascal LeclairThe University of British Columbia, A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE DEGREE OF MASTER OF SCIENCE in The Faculty of Graduate and Postdoctoral Studies (Cell and .